After that, hyphae of those isolates were cultured in potato dextrose broth (PDB) for DNA extraction. In addition, 5 isolates (R1, R2, R3, R4, and R5) were isolated from S. cajuputi in Tsunami-affected areas and 5 isolates (W1, W2, W3. Four isolates (T1, T2, T3, and T4) were isolated from M. Then, they were incubated on potato dextrose agar (PDA) at RT for 7 days. For the first method, pieces of 1x1 cm2 were cut from leaves and were surface sterilized. Cultured endophytic fungi were identified and compared by 2 methods: 1) morphology and 2) partial nucleotide sequence analysis of internal transcribed spacer (ITS) regions of 18S ribosomal DNA. cajuputi in Tsunami-affected and non-Tsunami-affected areas and in a leaf of S. From the above, we assumed that there might be different species of endophytic fungi in a leaf of M. Due to the survey of biodiversity destruction at Lampee-Had Tai Muang national park, Pang-nga, it was very interesting that Melaleuca cajuputi Powell could survive in Tsunami-affected area but not other plants including Syzygium gratum (Wight) S.N. In 2003, Tsunami destroyed a lot of natural resources along the western coast in 6 provinces of Thailand. Results will be presented describing the effect of application of the laccase-mediator system on the optical and mechanical properties of the prepared handsheets. Following the aging phase, the handsheets were subject to several chemical (viscosity and index Kappa) and physico-mechanical (colour, tensile breaking strength, stretch and the bursting strength) tests in order to characterize the effect of the aging conditions. In the first, the moisture varied (60, 80 and 100%), while the temperature was held constant (25 ◦C) in the second the temperature varied (60, 70 and 80 ◦C) and the moisture was held constant (50%). In order to evaluate the effect of moisture cycles and temperature, two aging sequences were used for each type of handsheet. The prepared handsheets were subject to an aging sequence in three different chambers: ultraviolet radiation (wavelength of 280 nm), temperature (19 ◦C) and moisture (70%) and thick saline fog at a concentration of 1% and temperature of 35 ◦C. Several types of handsheets were produced with 2 different grammage namely, 60 and 180 g/m2. The pulps used were (1) unbleached pine pulp from Portucel Tejo (2) unbleached eucalyptus pulp from Portucel Setubal (3) bleached eucalyptus pulp from Portucel Setubal and (4) pulp made from recycled paper from Renova S.A. Industrial pulps were obtained from different Portuguese pulp and paper companies.
Ewag osculator grinder oil plus#
The work presented describes a study on the effect of applying ligninolytic enzymes, such as a laccase plus mediator system, on a variety of different types of pine and eucalyptus pulps and subsequently subjecting these to different ageing processes. Microbial agents have been applied to the different stages of pulp and paper processing.
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